Molecular Mechanisms of Protein Quality Control

Our group studies complex proteases and chaperones that function as safeguards in the cell, preventing other proteins from undergoing dangerous interactions. By addressing mechanistic details of the protein-quality-control machinery, we aim to develop strategies against misbehaving proteins connected with neurodegenerative diseases, inclusion body myopathies, cancer and ageing. Moreover, differences in the protein folding and protein degradation pathways between bacterial pathogens and their hosts will guide the development of novel antibiotics. To this end, our studies concentrate on the following topics:

* Molecular machines rescuing damaged proteins

* Regulatory proteolysis in cellular signaling

* pArg and the targeted degradation of proteins

* Assembly and quality-control of myofilaments

To characterize the mechanistic properties of the proteins of interest, we apply an integrative approach combining methods from structural biology, protein biochemistry and molecular cell biology. Key expertises of our lab comprise

* Reconstitution of intricate folding and degradation pathways in vitro

* Biochemical trapping of transient protein-protein interactions

* Structural Biology of large, highly dynamic protein machines

* Phospho proteomics and cross-linking coupled mass spectrometry