Hellerschmied, D., Roessler, M., Lehner, A., Gazda, L., Stejskal, K., Imre, R., Mechtler, K., Dammermann, A., Clausen, T. (2018) UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins, Nat Commun, 9: 484 (abstract)


Ouyang, M., Li, X., Zhao, S., Pu, H., Shen, J., Adam, Z., Clausen, T., Zhang, L. (2017) The crystal structure of Deg9 reveals a novel octameric-type HtrA protease. Nat Plants, 3(12):973-982 (abstract)


Trentini, DB., Suskiewicz, MJ., Heuck, A., Kurzbauer, R., Deszcz, L., Mechtler, K., Clausen, T. (2016). Arginine phosphorylation marks proteins for degradation by a Clp protease. Nature. 539(7627):48-53 (abstract)

Heuck, A., Schitter-Sollner, S., Suskiewicz, MJ., Kurzbauer, R., Kley, J., Schleiffer, A., Rombaut, P., Herzog, F., Clausen, T. (2016). Structural basis for the disaggregase activity and regulation of Hsp104. Elife. 5 (abstract)

Suskiewicz, MJ., Clausen, T. (2016). Chemical Biology Interrogates Protein Arginine Phosphorylation. Cell Chem Biol. 23(8):888-90 (abstract)

Gallego, LD., Ghodgaonkar Steger, M., Polyansky, AA., Schubert, T., Zagrovic, B., Zheng, N., Clausen, T., Herzog, F., Köhler, A. (2016). Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6-Bre1. Proc Natl Acad Sci U S A. 113(38):10553-8 (abstract)


Poepsel, S., Sprengel, A., Sacca, B., Kaschani, F., Kaiser, M., Gatsogiannis, C., Raunser, S., Clausen, T., Ehrmann, M. (2015). Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nat Chem Biol. 11: 862-9 (abstract)

Schneider, M., Hellerschmied, D., Schubert, T., Amlacher, S., Vinayachandran, V., Reja, R., Pugh, BF., Clausen, T., Köhler, A. (2015). The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression. Cell. 162(5):1016-28 (abstract)


Schmidt, A., Trentini, DB., Spiess, S., Fuhrmann, J., Ammerer, G., Mechtler, K., Clausen, T. (2014). Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response. Mol Cell Proteomics. 13(2):537-50 (abstract)

Trentini, DB., Fuhrmann, J., Mechtler, K., Clausen, T. (2014). Chasing phosphoarginine proteins: development of a selective enrichment method using a phosphatase trap. Mol Cell Proteomics. 13: 1953-1964 (abstract)

Dikfidan, A., Loll, B., Zeymer, C., Magler, I., Clausen, T., Meinhart, A. (2014). RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1. Mol Cell. 54(6):975-86 (abstract)

Norell, D., Heuck, A., Tran-Thi, TA., Götzke, H., Jacob-Dubuisson, F., Clausen, T., Daley, DO., Braun, V., Müller, M., Fan, E. (2014). Versatile in vitro system to study translocation and functional integration of bacterial outer membrane proteins. Nat Commun. 5:5396 (abstract)

Hellerschmied, D., Clausen, T. (2014). Myosin chaperones. Curr Opin Struct Biol. 25:9-15 (abstract)

Karaca, E., Weitzer, S., Pehlivan, D., Shiraishi, H., Gogakos, T., Hanada, T., Jhangiani, SN., Wiszniewski, W., Withers, M., Campbell, IM., Erdin, S., Isikay, S., Franco, LM., Gonzaga-Jauregui, C., Gambin, T., Gelowani, V., Hunter, JV., Yesil, G., Koparir, E., Yilmaz, S., Brown, M., Briskin, D., Hafner, M., Morozov, P., Farazi, TA., Bernreuther, C., Glatzel, M., Trattnig, S., Friske, J., Kronnerwetter, C., Bainbridge, MN., Gezdirici, A., Seven, M., Muzny, DM., Boerwinkle, E., Ozen, M., Clausen, T., Tuschl, T., Yuksel, A., Hess, A., Gibbs, RA., Martinez, J., Penninger, JM., Lupski, JR. (2014). Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function. Cell. 157(3):636-50 (abstract)


Mastny, M., Heuck, A., Kurzbauer, R., Heiduk, A., Boisguerin, P., Volkmer, R., Ehrmann, M., Rodrigues, CD., Rudner, DZ., Clausen, T. (2013). CtpB Assembles a Gated Protease Tunnel Regulating Cell-Cell Signaling during Spore Formation in Bacillus subtilis. Cell. 155(3):647-58 (abstract)

Fuhrmann, J., Mierzwa, B., Trentini, DB., Spiess, S., Lehner, A., Charpentier, E., Clausen, T. (2013). Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in gram-positive bacteria. Cell Rep. 3(6):1832-9 (abstract)

Gazda, L., Pokrzywa, W., Hellerschmied, D., Löwe, T., Forné, I., Mueller-Planitz, F., Hoppe, T., Clausen, T. (2013). The Myosin Chaperone UNC-45 Is Organized in Tandem Modules to Support Myofilament Formation in C. elegans. Cell. 152(1-2):183-95 (abstract)

Hellerschmied, D., Clausen, T. (2013). Myosin chaperones. Curr Opin Struct Biol. 25C:9-15 (abstract)

Malvezzi, F., Litos, G., Schleiffer, A., Heuck, A., Mechtler, K., Clausen, T., Westermann, S. (2013). A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors. EMBO J. (abstract)


Breuss, M., Heng, JI., Poirier, K., Tian, G., Jaglin, XH., Qu, Z., Braun, A., Gstrein, T., Ngo, L., Haas, M., Bahi-Buisson, N., Moutard, ML., Passemard, S., Verloes, A., Gressens, P., Xie, Y., Robson, KJ., Rani, DS., Thangaraj, K., Clausen, T., Chelly, J., Cowan, NJ., Keays, DA. (2012). Mutations in the ?-Tubulin Gene TUBB5 Cause Microcephaly with Structural Brain Abnormalities. Cell Rep. 2(6):1554-62 (abstract)

Dal Santo, S., Stampfl, H., Krasensky, J., Kempa, S., Gibon, Y., Petutschnig, E., Rozhon, W., Heuck, A., Clausen, T., Jonak, C. (2012). Stress-Induced GSK3 Regulates the Redox Stress Response by Phosphorylating Glucose-6-Phosphate Dehydrogenase in Arabidopsis. Plant Cell. 24(8):3380-92 (abstract)

Malet, H., Canellas, F., Sawa, J., Yan, J., Thalassinos, K., Ehrmann, M., Clausen, T., Saibil, HR. (2012). Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ. Nat Struct Mol Biol. 19(2):152-7 (abstract)

Tennstaedt, A., Pöpsel, S., Truebestein, L., Hauske, P., Brockmann, A., Schmidt, N., Irle, I., Sacca, B., Niemeyer, CM., Brandt, R., Ksiezak-Reding, H., Tirniceriu, AL., Egensperger, R., Baldi, A., Dehmelt, L., Kaiser, M., Huber, R., Clausen, T., Ehrmann, M. (2012). Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates. J Biol Chem. 287(25):20931-41 (abstract)


Clausen, T., Kaiser, M., Huber, R., Ehrmann, M. (2011). HTRA proteases: regulated proteolysis in protein quality control. Nat Rev Mol Cell Biol. 12(3):152-62 (abstract)

Heuck, A., Schleiffer, A., Clausen, T. (2011). Augmenting ?-augmentation: structural basis of how BamB binds BamA and may support folding of outer membrane proteins. J Mol Biol. 406(5):659-66 (abstract)

Kley, J., Schmidt, B., Boyanov, B., Stolt-Bergner, PC., Kirk, R., Ehrmann, M., Knopf, RR., Naveh, L., Adam, Z., Clausen, T. (2011). Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure. Nat Struct Mol Biol. 18(6):728-31 (abstract)

Merdanovic, M., Clausen, T., Kaiser, M., Huber, R., Ehrmann, M. (2011). Protein quality control in the bacterial periplasm. Annu Rev Microbiol. 65:149-68 (abstract)

Sawa, J., Malet, H., Krojer, T., Canellas, F., Ehrmann, M., Clausen, T. (2011). Molecular adaptation of the DegQ protease to exert protein quality control in the bacterial cell envelope. J Biol Chem. 286(35):30680-90 (abstract)

Truebestein, L., Tennstaedt, A., Mönig, T., Krojer, T., Canellas, F., Kaiser, M., Clausen, T., Ehrmann, M. (2011). Substrate-induced remodeling of the active site regulates human HTRA1 activity. Nat Struct Mol Biol. 18(3):386-8 (abstract)


Hasenbein, S., Meltzer, M., Hauske, P., Kaiser, M., Huber, R., Clausen, T., Ehrmann, M. (2010). Conversion of a regulatory into a degradative protease. J Mol Biol. 397(4):957-66 (abstract)

Krojer, T., Sawa, J., Huber, R., Clausen, T. (2010). HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues. Nat Struct Mol Biol. 17(7):844-52 (abstract)

Sawa, J., Heuck, A., Ehrmann, M., Clausen, T. (2010). Molecular transformers in the cell: lessons learned from the DegP protease-chaperone. Curr Opin Struct Biol. 20(2):253-8 (abstract)


Breithaupt, C., Kurzbauer, R., Schaller, F., Stintzi, A., Schaller, A., Huber, R., Macheroux, P., Clausen, T. (2009). Structural basis of substrate specificity of plant 12-oxophytodienoate reductases. J Mol Biol. 392(5):1266-77 (abstract)

Fuhrmann, J., Schmidt, A., Spiess, S., Lehner, A., Turgay, K., Mechtler, K., Charpentier, E., Clausen, T. (2009). McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR. Science. 324(5932):1323-7 (abstract) (fulltext)

Hauske, P., Mamant, N., Hasenbein, S., Nickel, S., Ottmann, C., Clausen, T., Ehrmann, M., Kaiser, M. (2009). Peptidic small molecule activators of the stress sensor DegS. Mol Biosyst. 5(9):980-5 (abstract)

Hauske, P., Meltzer, M., Ottmann, C., Krojer, T., Clausen, T., Ehrmann, M., Kaiser, M. (2009). Selectivity profiling of DegP substrates and inhibitors. Bioorg Med Chem. 17(7):2920-4 (abstract)

Meltzer, M., Hasenbein, S., Mamant, N., Merdanovic, M., Poepsel, S., Hauske, P., Kaiser, M., Huber, R., Krojer, T., Clausen, T., Ehrmann, M. (2009). Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli. Res Microbiol. 160(9):660-6 (abstract)


Krojer, T., Pangerl, K., Kurt, J., Sawa, J., Stingl, C., Mechtler, K., Huber, R., Ehrmann, M., Clausen, T. (2008). Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins. Proc Natl Acad Sci U S A. 105(22):7702-7 (abstract)

Krojer, T., Sawa, J., Schäfer, E., Saibil, HR., Ehrmann, M., Clausen, T. (2008). Structural basis for the regulated protease and chaperone function of DegP. Nature. 453(7197):885-90 (abstract)

Meltzer, M., Hasenbein, S., Hauske, P., Kucz, N., Merdanovic, M., Grau, S., Beil, A., Jones, D., Krojer, T., Clausen, T., Ehrmann, M., Kaiser, M. (2008). Allosteric activation of HtrA protease DegP by stress signals during bacterial protein quality control. Angew Chem Int Ed Engl. 47(7):1332-4 (abstract)


Hasselblatt, H., Kurzbauer, R., Wilken, C., Krojer, T., Sawa, J., Kurt, J., Kirk, R., Hasenbein, S., Ehrmann, M., Clausen, T. (2007). Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stress. Genes Dev. 21(20):2659-70 (abstract)


Breithaupt, C., Kurzbauer, R., Lilie, H., Schaller, A., Strassner, J., Huber, R., Macheroux, P., Clausen, T. (2006). Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization. Proc Natl Acad Sci U S A. 103(39):14337-42 (abstract)

Grau, S., Richards, PJ., Kerr, B., Hughes, C., Caterson, B., Williams, AS., Junker, U., Jones, SA., Clausen, T., Ehrmann, M. (2006). The role of human HtrA1 in arthritic disease. J Biol Chem. 281(10):6124-9 (abstract)


Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., Richards, P., Jones, SA., Shridhar, V., Clausen, T., Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proc Natl Acad Sci U S A. 102(17):6021-6 (abstract)

Groll, M., Bochtler, M., Brandstetter, H., Clausen, T., Huber, R. (2005). Molecular machines for protein degradation. Chembiochem. 6(2):222-56 (abstract)

Kitzing, K., Fitzpatrick, TB., Wilken, C., Sawa, J., Bourenkov, GP., Macheroux, P., Clausen, T. (2005). The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes. J Biol Chem. 280(30):27904-13 (abstract)


Ehrmann, M., Clausen, T. (2004). Proteolysis as a regulatory mechanism. Annu Rev Genet. 38:709-24 (abstract)

Fitzpatrick, TB., Auweter, S., Kitzing, K., Clausen, T., Amrhein, N., Macheroux, P. (2004). Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation. Protein Expr Purif. 36(2):280-91 (abstract)

Kurzbauer, R., Teis, D., de Araujo, ME., Maurer-Stroh, S., Eisenhaber, F., Bourenkov, GP., Bartunik, HD., Hekman, M., Rapp, UR., Huber, LA., Clausen, T. (2004). Crystal structure of the p14/MP1 scaffolding complex: how a twin couple attaches mitogen-activated protein kinase signaling to late endosomes. Proc Natl Acad Sci U S A. 101(30):10984-9 (abstract)

Wilken, C., Kitzing, K., Kurzbauer, R., Ehrmann, M., Clausen, T. (2004). Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease. Cell. 117(4):483-94 (abstract)


Groll, M., Clausen, T. (2003). Molecular shredders: how proteasomes fulfill their role. Curr Opin Struct Biol. 13(6):665-73 (abstract)

Kaiser, JT., Bruno, S., Clausen, T., Huber, R., Schiaretti, F., Mozzarelli, A., Kessler, D. (2003). Snapshots of the cystine lyase C-DES during catalysis. Studies in solution and in the crystalline state. J Biol Chem. 278(1):357-65 (abstract)


Bertoldi, M., Cellini, B., Clausen, T., Voltattorni, CB. (2002). Spectroscopic and kinetic analyses reveal the pyridoxal 5'-phosphate binding mode and the catalytic features of Treponema denticola cystalysin. Biochemistry. 41(29):9153-64 (abstract)

Clausen, T., Southan, C., Ehrmann, M. (2002). The HtrA family of proteases: implications for protein composition and cell fate. Mol Cell. 10(3):443-55 (abstract)

Garrido-Franco, M., Ehlert, S., Messerschmidt, A., Marinkovic', S., Huber, R., Laber, B., Bourenkov, GP., Clausen, T. (2002). Structure and function of threonine synthase from yeast. J Biol Chem. 277(14):12396-405 (abstract)

Garrido-Franco, M., Laber, B., Huber, R., Clausen, T. (2002). Enzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis. J Mol Biol. 321(4):601-12 (abstract)

Krojer, T., Garrido-Franco, M., Huber, R., Ehrmann, M., Clausen, T. (2002). Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine. Nature. 416(6879):455-9 (abstract)


Breithaupt, C., Strassner, J., Breitinger, U., Huber, R., Macheroux, P., Schaller, A., Clausen, T. (2001). X-ray structure of 12-oxophytodienoate reductase 1 provides structural insight into substrate binding and specificity within the family of OYE. Structure. 9(5):419-29 (abstract)

Breitinger, U., Clausen, T., Ehlert, S., Huber, R., Laber, B., Schmidt, F., Pohl, E., Messerschmidt, A. (2001). The three-dimensional structure of cystathionine beta-lyase from Arabidopsis and its substrate specificity. Plant Physiol. 126(2):631-42 (abstract)

Franco, MG., Laber, B., Huber, R., Clausen, T. (2001). Structural basis for the function of pyridoxine 5'-phosphate synthase. Structure. 9(3):245-53 (abstract)

Steegborn, C., Danot, O., Huber, R., Clausen, T. (2001). Crystal structure of transcription factor MalT domain III: a novel helix repeat fold implicated in regulated oligomerization. Structure. 9(11):1051-60 (abstract)

Steegborn, C., Laber, B., Messerschmidt, A., Huber, R., Clausen, T. (2001). Crystal structures of cystathionine gamma-synthase inhibitor complexes rationalize the increased affinity of a novel inhibitor. J Mol Biol. 311(4):789-801 (abstract)


Clausen, T., Kaiser, JT., Steegborn, C., Huber, R., Kessler, D. (2000). Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis. Proc Natl Acad Sci U S A. 97(8):3856-61 (abstract)

Clausen, T., Schlegel, A., Peist, R., Schneider, E., Steegborn, C., Chang, YS., Haase, A., Bourenkov, GP., Bartunik, HD., Boos, W. (2000). X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression. EMBO J. 19(5):831-42 (abstract)

Garrido Franco, M., Huber, R., Schmidt, FS., Laber, B., Clausen, T. (2000). Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme. Acta Crystallogr D Biol Crystallogr. 56(Pt 8):1045-8 (abstract)

Kaiser, JT., Clausen, T., Bourenkow, GP., Bartunik, HD., Steinbacher, S., Huber, R. (2000). Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly. J Mol Biol. 297(2):451-64 (abstract)

Krupka, HI., Huber, R., Holt, SC., Clausen, T. (2000). Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme. EMBO J. 19(13):3168-78 (abstract)

Schreiber, V., Steegborn, C., Clausen, T., Boos, W., Richet, E. (2000). A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors. Mol Microbiol. 35(4):765-76 (abstract)

Steegborn, C., Clausen, T. (2000). Specific inhibition of transsulfuration enzymes. Rec Res Developm Biochem. 2:191-207


Clausen, T., Wahl, MC., Messerschmidt, A., Huber, R., Fuhrmann, JC., Laber, B., Streber, W., Steegborn, C. (1999). Cloning, purification and characterisation of cystathionine gamma-synthase from Nicotiana tabacum. Biol Chem. 380(10):1237-42 (abstract)

Laber, B., Maurer, W., Hanke, C., Gräfe, S., Ehlert, S., Messerschmidt, A., Clausen, T. (1999). Characterization of recombinant Arabidopsis thaliana threonine synthase. Eur J Biochem. 263(1):212-21 (abstract)

Steegborn, C., Clausen, T., Sondermann, P., Jacob, U., Worbs, M., Marinkovic, S., Huber, R., Wahl, MC. (1999). Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration. J Biol Chem. 274(18):12675-84 (abstract)

Steegborn, C., Messerschmidt, A., Laber, B., Streber, W., Huber, R., Clausen, T. (1999). The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity. J Mol Biol. 290(5):983-96 (abstract)


Clausen, T., Huber, R., Prade, L., Wahl, MC., Messerschmidt, A. (1998). Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution. EMBO J. 17(23):6827-38 (abstract)


Clausen, T., Huber, R., Messerschmidt, A., Pohlenz, HD., Laber, B. (1997). Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study. Biochemistry. 36(41):12633-43 (abstract)

Clausen, T., Laber, B., Messerschmidt, A. (1997). Mode of action of cystathionine beta-lyase. Biol Chem. 378(3-4):321-6 (abstract)

Jacob, U., Mack, M., Clausen, T., Huber, R., Buckel, W., Messerschmidt, A. (1997). Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases. Structure. 5(3):415-26 (abstract)

Wahl, MC., Huber, R., Prade, L., Marinkovic, S., Messerschmidt, A., Clausen, T. (1997). Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli. FEBS Lett. 414(3):492-6 (abstract)


Clausen, T., Huber, R., Laber, B., Pohlenz, HD., Messerschmidt, A. (1996). Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A. J Mol Biol. 262(2):202-24 (abstract)

Laber, B., Clausen, T., Huber, R., Messerschmidt, A., Egner, U., Müller-Fahrnow, A., Pohlenz, HD. (1996). Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase. FEBS Lett. 379(1):94-6 (abstract)


Stenberg, K., Clausen, T., Lindqvist, Y., Macheroux, P. (1995). Involvement of Tyr24 and Trp108 in substrate binding and substrate specificity of glycolate oxidase. Eur J Biochem. 228(2):408-16 (abstract)